i scei expression plasmid (Addgene inc)
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Addgene inc
i scei expression plasmid
I Scei Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/i+scei+plasmid/pCBASceI+(Plasmid+%2326477)/bio_rxiv__64898__2026__02__17__706442-228-11-14
Average 96 stars, based on 298 article reviews
I Scei Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/i+scei+plasmid/pCBASceI+(Plasmid+%2326477)/bio_rxiv__64898__2026__02__17__706442-228-11-14
Average 96 stars, based on 298 article reviews
i scei expression plasmid - by Bioz Stars,
2026-11
96/100 stars
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Transfection:Article Title: Oncogenic Impact of TONSL, a Homologous Recombination Repair Protein at the Replication Fork, in Cancer Stem Cells. Article Snippet: .. The selected clone was then plated in a 6-well plate and transfected with 1 μg of Article Title: DNA double-strand break-capturing nuclear envelope tubules drive DNA repair. Article Snippet: .. Cells were re-seeded, and, 48 h after siRNA transfection, cells were transfected with 1 μg of Article Title: Oncogenic Impact of TONSL, a Homologous Recombination Repair Protein at the Replication Fork, in Cancer Stem Cells Article Snippet: .. The selected clone was then plated in a 6-well plate and transfected with 1 μg of Article Title: USP22 interacts with PALB2 and promotes chemotherapy resistance via homologous recombination of DNA double strand breaks Article Snippet: .. Briefly, cells were transfected with the siRNA described above using RNAiMax, followed, 24 hr later, by co-transfection using lipofectamine-2000 of the Plasmid Preparation:Article Title: Oncogenic Impact of TONSL, a Homologous Recombination Repair Protein at the Replication Fork, in Cancer Stem Cells. Article Snippet: .. The selected clone was then plated in a 6-well plate and transfected with 1 μg of Article Title: DNA double-strand break-capturing nuclear envelope tubules drive DNA repair. Article Snippet: .. Cells were re-seeded, and, 48 h after siRNA transfection, cells were transfected with 1 μg of Article Title: Oncogenic Impact of TONSL, a Homologous Recombination Repair Protein at the Replication Fork, in Cancer Stem Cells Article Snippet: .. The selected clone was then plated in a 6-well plate and transfected with 1 μg of Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: .. ΔMDC1 DR-GFP knock-in cells were nucleofected using a Lonza 4D nucleofector in RPMI plus 50 mM HEPES with 400 ng Article Title: USP22 interacts with PALB2 and promotes chemotherapy resistance via homologous recombination of DNA double strand breaks Article Snippet: .. Briefly, cells were transfected with the siRNA described above using RNAiMax, followed, 24 hr later, by co-transfection using lipofectamine-2000 of the Article Title: Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide. Article Snippet: widely utilized for increased yield and solubility of recombinant proteins.. However, the production of additional large fusion partners poses an additional burden to the host, leading to a decreased protein yield.. In this study, we identified two highly disordered short peptides that were able to increase the solubility of an artificially engineered aggregationprone protein, GFP-GFIL4, from 0.6% to 61% (D3-DP00592) and 46% (D4-DP01038) selected from DisProt database. Article Title: The immediate-early protein 1 of human herpesvirus 6B interacts with NBS1 and inhibits ATM signaling. Article Snippet: .. After 24 h, cells were co-transfected with 900 ng of the other:Article Title: The immediate-early protein 1 of human herpesvirus 6B interacts with NBS1 and inhibits ATM signaling Article Snippet: About 15,000 cells subjected to NBS1 or control RNAi were added to a 96-well plate and cell viability was evaluated by CellTiter-Glo Cell Viability Assay (Promega) according to the manufacturer’s instructions. Knock-In:Article Title: The PST repeat region of MDC1 is a tunable multivalent chromatin tethering domain Article Snippet: .. ΔMDC1 DR-GFP knock-in cells were nucleofected using a Lonza 4D nucleofector in RPMI plus 50 mM HEPES with 400 ng Polymerase Chain Reaction:Article Title: Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide. Article Snippet: widely utilized for increased yield and solubility of recombinant proteins.. However, the production of additional large fusion partners poses an additional burden to the host, leading to a decreased protein yield.. In this study, we identified two highly disordered short peptides that were able to increase the solubility of an artificially engineered aggregationprone protein, GFP-GFIL4, from 0.6% to 61% (D3-DP00592) and 46% (D4-DP01038) selected from DisProt database. Clone Assay:Article Title: Enhancement of the solubility of recombinant proteins by fusion with a short-disordered peptide. Article Snippet: widely utilized for increased yield and solubility of recombinant proteins.. However, the production of additional large fusion partners poses an additional burden to the host, leading to a decreased protein yield.. In this study, we identified two highly disordered short peptides that were able to increase the solubility of an artificially engineered aggregationprone protein, GFP-GFIL4, from 0.6% to 61% (D3-DP00592) and 46% (D4-DP01038) selected from DisProt database. Negative Control:Article Title: The immediate-early protein 1 of human herpesvirus 6B interacts with NBS1 and inhibits ATM signaling. Article Snippet: .. After 24 h, cells were co-transfected with 900 ng of the |
